Legend of the Guardians: The Owls of Ga'Hoole is a video game for Nintendo Wii, Nintendo DS/DSi/DSiXL, Playstation 3, Xbox 360/Live based on a film of the same name. The game takes place at the same time the movie takes place. It focuses on a young owl named Shard, who embarks on a journey across the world of Ga'Hoole to uncover the truth about the evil Pure Ones and stop their plans.
A young owlet named Shard is born to his parents. One night, though, An evil organization of owls called the Pure Ones attack the nest and kill Shard's parents, but the Guardians swoop in and rescue the infant Shard before he could be killed like his parents and carried him safely to the Great Ga'Hoole tree where Shard is raised. Many years later, Shard embarks on a quest to uncover the Pure Ones's true intentions and save Ga'Hoole.
- Shard - A young guardian and the main protagonist in the game. His parents were killed by the Pure Ones when he was a hatchling, but he was rescued by the Guardians. Years later, he embarks on a quest to uncover the Pure Ones's intentions. He is often accompanied by a Spotted Owl named Parzival and is a student of the guardian Allomere who is secretly a pure one.
- Parzival - A Spotted owl young guardian and friend of Shard. He is considered to be the game's secondary protagonist. He often accompanies Shard on his quest to help defeat the Pure Ones as his wingman.
- Ezylryb - An Whiskered screech owl elder guardian who was known throughout Ga'Hoole as the legendary hero Lyze of Kiel. He is also the one who gives Shard his quest.
- Streak - An eagle who lives in the desert of Kuneer. He is captured by bats while investigating the disappearance of owlets at the hands of the Pure Ones. After Shard and Parzival free him, he becomes one of Shard's wingmen and helps defeat the bats.
- Grettir - The father of Shard and retired acolyte. He is killed along with his mate by Allomere.
- Lygeia - The mother of Shard and mate of Grettir. She is killed along with her mate by Allomere.
- Bryony - A female Barn Owl and leader of the Resistance against the Pure Ones stationed in the forest kingdom of Tyto. She is one of Shard's wingmen and helps him wipe out the Pure Ones in Tyto.
- Cormac - A Barn Owl hermit who lives in the Glauxian Brothers Retreat. He is one of Shard's wingmen and helps Shard and Parzival save young monks from the Pure Ones and the Hagsfiends.
- Allomere - A Great Gray Owl guardian and leader of the search-and-rescue guardians who betrayed the Guardians to the Pure Ones and is the main antagonist in the game. He is responsible for the deaths of Shard's parents Grettir and Lygeia. After Shard destroys the Pure Ones's forge in the Beaks, Shard and Allomere clash face-to-face which leads to Allomere being killed by rocks with Shard looking at his mask in sorrow.
- Uriah - A Great Horned owl hermit who was due to be fed on. He becomes one of Shard's wingmen after he saves him.
- Bat Chieftain - The leader of the bats.He is defeated by Shard when he hit him with hot coals.
- Hagsfiend Queen - She is the leader of hagsfiends and has the ability to create fog. Every few seconds, the player must defeat 3 fleck bats to get rid of her barrier.